Iron-regulatory proteins secure iron availability in cardiomyocytes to prevent heart failure
- SabaHaddad
- YongWang
- BrunoGaly
- MortimerKorf-Klingebiel
- ValentinHirsch
- Abdul M.Baru
- FatemehRostami
- Marc R.Reboll
- JörgHeineke
- UlrichFlögel
- StephanieGroos
- AndréRenner
- KarlToischer
- FabianZimmermann
- StefanEngeli
- JensJordan
- JohannBauersachs
- Matthias W.Hentze
- Kai C.Wollert
- TiborKempf
Results
Reduced iron content, IRE binding activity, and transferrin receptor expression in the failing human heart
library(math)
labels(data) <- c("non-failing heart (NF)", "failing heart (F)")
library(reticulate)
np <- import("numpy", convert=FALSE)
(x <- np$arange(1, 9)$reshape(2L, 2L))
## [[ 99. 52.]
## [ 96. 40.]
## [ 100. 38.]
## [ 105. 18.]
## [ np.nan. 11.]
## [ np.nan. 5.]
## [ np.nan. 42.]
## [ np.nan. 55.]
## [ np.nan 53.]
## [ np.nan 39.]
## [ np.nan. 42.]
## [ np.nan 50.]
## [ 23. 24.]]
df = pd.DataFrame.from_records(data, columns=labels)
tt = ttest_ind(df['non-failing heart (NF)'],
df['failing heart (F)'],
equal_var=False, nan_policy='omit')
pvalue = tt.pvalue
glue('pvalue', math.ceil(pvalue * 1000.0) / 1000.0)
pvalue <- 0.00152894
Consistent with previous
reports {cite}maeder2011,leszek2012
iron
concentration was significantly lower in LV tissue samples from patients with advanced
heart failure than in LV tissue samples from unused donor hearts. As shown by
electrophoretic mobility shift assays, IRE binding activity was significantly (p <
formatC(pvalue,3,format="f")
Figure 1<fig1>
). Protein
expression levels of the transferrin receptor were significantly lower in failing hearts
than in the controls.
fig, ax = plt.subplots()
d = df.to_numpy()
f = [d[m] for d, m in zip(d.T, ~np.isnan(d).T)]
ax.boxplot(f)
ax.set_ylim([0, 150])
ax.set_ylabel('IRE binding activity (%)', fontsize=14)
ax.set_xticklabels(['NF', 'F'])
ax.tick_params(axis='x', labelsize=14, labelrotation=45)
ax.tick_params(axis='y', labelsize=14)
glue('fig1', fig, display=False)
References
LSS+12
Przemysław Leszek, Barbara Sochanowicz, Małgorzata Szperl, Piotr Kolsut, Kamil Brzóska, Walerian Piotrowski, Tomasz M. Rywik, Bożena Danko, Halina Polkowska-Motrenko, Jacek M. Różański, and et al. Myocardial iron homeostasis in advanced chronic heart failure patients. International Journal of Cardiology, 159(1):47–52, Aug 2012. URL: https://doi.org/10.1016/j.ijcard.2011.08.006, doi:10.1016/j.ijcard.2011.08.006. #### MKdRK11 Micha T. Maeder, Ouda Khammy, Cris dos Remedios, and David M. Kaye. Myocardial and systemic iron depletion in heart failure. Journal of the American College of Cardiology, 58(5):474–480, Jul 2011. URL: https://doi.org/10.1016/j.jacc.2011.01.059, doi:10.1016/j.jacc.2011.01.059.