Iron-regulatory proteins secure iron availability in cardiomyocytes to prevent heart failure

Results

Reduced iron content, IRE binding activity, and transferrin receptor expression in the failing human heart

labels <- list('non-failing heart (NF)', 'failing heart (F)')

data <- list(c(99, 52), c(96, 40), c(100, 38), c(105, 18), 
             c(NA_integer_, 11), c(NA_integer_, 5), c(NA_integer_, 42), 
             c(NA_integer_, 55), c(NA_integer_, 53), c(NA_integer_, 39),
             c(NA_integer_, 42), c(NA_integer_, 50))
head(data)

df <- setNames(do.call(rbind.data.frame, 
                       lapply(data, function(d) data.frame(d[1], d[2]))),
              labels)    

                           
                           
results <- t.test(df[['non-failing heart (NF)']], df[['failing heart (F)']])


                           
results$statistic
results$estimate
results$p.value
                           

Consistent with previous reports MKdRK11 LSS+12 iron concentration was significantly lower in LV tissue samples from patients with advanced heart failure than in LV tissue samples from unused donor hearts. As shown by electrophoretic mobility shift assays, IRE binding activity was significantly (p < ceiling(results$p.value * 1000.0)/ 1000.0) reduced in failing hearts (most pronounced in patients with ischemic cardiomyopathy)(). Protein expression levels of the transferrin receptor were significantly lower in failing hearts than in the controls.


df$NF <- df$`non-failing heart (NF)`
df$F <- df$`failing heart (F)`
boxplot(df[3:4],
        data=df,
        cex.lab=0.65,
        xlab="Fig. 1 IRE binding activity for non-failing (NF) and failing (F) hearts.",
        ylab="IRE binding activity (%)",
        col="orange",
        border="brown",
        ylim = c(0, 120)
)

References

LSS+12

Przemysław Leszek, Barbara Sochanowicz, Małgorzata Szperl, Piotr Kolsut, Kamil Brzóska, Walerian Piotrowski, Tomasz M. Rywik, Bożena Danko, Halina Polkowska-Motrenko, Jacek M. Różański, and et al. Myocardial iron homeostasis in advanced chronic heart failure patients. International Journal of Cardiology, 159(1):47–52, Aug 2012. URL: https://doi.org/10.1016/j.ijcard.2011.08.006, doi:10.1016/j.ijcard.2011.08.006.

MKdRK11

Micha T. Maeder, Ouda Khammy, Cris dos Remedios, and David M. Kaye. Myocardial and systemic iron depletion in heart failure. Journal of the American College of Cardiology, 58(5):474–480, Jul 2011. URL: https://doi.org/10.1016/j.jacc.2011.01.059, doi:10.1016/j.jacc.2011.01.059.